Tube    
                                  
           
          A                       
          
B         
           C

Ratio (Parts Soln:Parts Diluent)    
           
_____________ _____________ _____________

Dilution

(compared to previous tube)
                     
_____________ _____________ _____________

Total Dilution

(compared to original
stock)                     
_____________ _____________ _____________

Cell Concentration
(cells/ml)                    
_____________ _____________ _____________

How many colonies on the plate?   _____________

You have a stock broth culture of
Staphylococcus epidermidis at a concentration of 5 x
10⁶ cells per ml. You perform the following serial
dilution and perform a spread plate using 100 ml of your last
dilution.

Use the table below to fill out the information for each tube.
(3 pts)

How many colonies would you get on the plate? (1 pt)