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Question: A polypeptide has the sequence:           &nbs…

by | Dec 4, 2023 | Posted Questions

A polypeptide has the sequence:

                               
Leu-Leu-Trp-Tyr-Ser-Glu

a.
        Calculate the molecular
weight of the polypeptide.

b.        
Assuming a molar absorptivity for Trp and Tyr of
3400M-1cm-1 and
1400M-1cm-1, respectively, estimate the
extinction coefficient for this peptide at a wavelength, l, of 280
nm.

c.
       
A solution of
this peptide is placed in a cuvette with a 1 cm pathlength and its
absorbance is found to be 1.3 at 280 nm. What is the concentration
of the polypeptide in mg/ml?

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