You have isolated four different mutants unable to synthesize compound E required
for growth. You know that compounds A through D are involved in the biosynthetic
pathway that produces compound E, but you do not know the order in which they are
synthesized by a wild-type .com/biology/the-cell-division-cylce”>cell. You supplement the growth of each of the 4 mutants
with each compound and assay its ability to support growth. Using the .com/computer-science/data”>data below,
construct a biochemical pathway indicating the order of the compounds and the step
where each .com/biology/mutation”>mutation is acting. Explain how you arrived at the map.

Compound added
A B C D E
Mutation
1 ? ? ? ? +
2 ? + + + +
3 ? ? + + +
4 ? ? + ? +

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