Experiment 1: Paper Chromatography
Data Tables and Post-Lab Assessment
Table 1:
Part 1: Chromatography Data
Solvent
Acetic Acid
Acetone Distance from Original Line to Solvent Front
Solvent: 49mm / Pigment: 11mm 1 Solvent: 51mm / Pigment: 36mm 2 Solvent: 55mm / Pigment: 0mm Mineral Oil
Water Number of Bands 1 Solvent: 51mm / Pigment: 13mm 1 Rf Factor
.2245
.7059
0
.2549 Table 2: Part 3: Photosynthesis Data
Test Tube Time Required to
Change Color Initial Color Final Color Time Required to
Change Color (Hours) 1
2
3 Post-Lab Questions
1. What did the different colored bands signify in each solvent for Part 1? What pigments can
you associate them with?
©eScience Labs, LLC 2013 The color bands signified the separation of the chlorophyll a (blue green) chlorophyll b
(green) and xanthrophyll (yellow). 2. What is the osmolarity fluid used in Part 2? Why is this important? Why is it essential to keep
it cool? The fluid used in Part 2 was Sucrose Solution, it had to be cold since the cold temperature
inhibits the rate of photosynthesis, therefore allowing protection to the proteins. ©eScience Labs, LLC 2013 3. How could you modify this experiment to show the effects of different wavelengths of light on
the photosynthetic rate? You could measure the oxygen produced by the leaves with each different relevant
wavelength and record the same. 4. Some plants (grasses) tend to contain a greater concentration of chlorophyll than others
(pines). Can you develop a hypothesis to explain this? Would it be testable? If grass and pines are independently tested for photosynthesis rate, then grass will be more
responsive. Testable. ©eScience Labs, LLC 2013
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