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Question: ΔCT ΔΔCT 2-ΔΔCT sample CD4 CT Average CD4 CT GAPDH CT Average GAPDH CT CD4 CT- GAPDH CT Δ…

ΔCT ΔΔCT 2-ΔΔCT
sample CD4 CT Average CD4 CT GAPDH CT Average GAPDH CT CD4 CT– GAPDH CT ΔCT tissue-
ΔCTliver
fold difference relative to liver
liver 32.10 31.89 17.35 17.16 14.73 0.00 1.00
31.69 16.96
thymus 24.09 24.11 15.91 16.04 8.06 -6.67 101.75
24.12 16.17
lymph nodes 32.35 32.17 17.60 17.72 14.45 -0.28 1.22
31.99 17.84
spleen 28.25 27.83 14.38 14.56 13.27 -1.46 2.75
27.41 14.73
bone marrow 27.96 28.18 14.15 14.18 14.00 -0.74 1.66
28.40 14.21

The values are replicated as you can see with the Two number
sets for each sample. How do you do the calculations and plot a
QRT-PCR Data analysis plots such as Linear amplification plot, log
view amplification plot, and representing each sample. Please show
all your calculation

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