1. A mutation occurs in the DNA within the sequence
that encodes region 4 of the attenuator. This mutation results in
an inability of region 4 to stably base pair with region 3. Would
you predict that this mutation would negatively impact trp operon
regulation in the presence of high [trp], in the presence of low
[trp], in both conditions, or in neither condition? Explain
why.
2. Draw and appropriately label a cartoon that illustrates what
would happen during trp operon regulation were the above mutation
in question #1 present. Your cartoon should include the DNA, mRNA,
ribosome, RNA polymerase, leader, attenuator, and the start of the
trp operon coding region (the trpE gene).
#2 ONLY ANSWER #2