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Question: 1. A plasmid has 3 restriction sites for a restriction enzyme. If the plasmid is digested with th…

1. A plasmid has 3 restriction sites for a restriction enzyme.
If the plasmid is digested with this restriction enzyme, how many
fragments will be generated? Note: in order to get to the right
answer please draw.

2. Would we be able to detect with electrophoresis a mutation in
hemoglobin that substituted amino acid Glu (glutamic acid) for
another amino acid, Asp (aspartic acid). Please justify your
answer. Hint: you will need to find and look at the chemical
structure of these two amino acids.

3. You’ve just finished cloning a DNA fragment into a plasmid,
like we did using paper models in lab 8. Please describe the
strategy to check for orientation of the insert within the plasmid.
No specific restriction sites should be mentioned, but instead
describe the location of the site (within the inserted sequence or
in the plasmid sequence).

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