1.
An experiment was performed to determine the effects of an
inhibitor on the breakdown of glycogen by an enzyme. In an
accompanying experiment, the inhibitor was added to the
glycogen-enzyme suspension and reacted using the same experimental
conditions. The data obtained from these experiments is tabulated
below.
|
Glycogen (mM) |
Product Formed Glycogen only (mM/min) |
Product Formed Glycogen and Inhibitor |
|
0.5 |
22.6 |
15.9 |
|
1.0 |
32.1 |
24.9 |
|
1.5 |
38.1 |
28.2 |
|
2.0 |
41.2 |
35.1 |
|
2.5 |
44.7 |
40.0 |
|
3.0 |
48.5 |
43.3 |
a.Draw the Michaelis-Menten and Lineweaver-Burke plots of these
data.
b.Determine the form of inhibition observed from these results,
and explain your rationale for this form.
c.Determine the values for Km and
Vmax from these results.
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