1. If you began with a sample that contained 8
copies of double-stranded DNA, how many copies would be present
after 35 cycles of PCR, assuming 100% efficiency?
2.
. In setting up a PCR reaction, which set of
primers below is able to amplify the target sequence (represented
as a series of asterisks)?
5¢-TTAAGATCCGTTACGTATGC**********AACCCGTTCCTACGAACCTT-3¢
3¢-AATTCTAGGCAATGCATACG**********TTGGGCAAGGATGCTTGGAA-5¢
SET 1: 5¢-TTAAGATCCGTT-3¢; 5¢-CGTTCCTACGAA-3¢
SET 2: 5¢-CGTATGCATTGC-3¢; 5¢-TTCCAAGCATCC-3¢
SET 3: 5¢-GATCCGTTACGT-3¢; 5¢-TTCGTAGGAACG-3¢