1. You’ve just finished cloning a DNA fragment into a
plasmid, Please describe the strategy to check
for orientation of the insert within the plasmid. No specific
restriction sites should be mentioned, but instead describe the
location of the site (within the inserted sequence or in the
plasmid sequence)
2. A plasmid has 3 restriction sites for a restriction enzyme.
If the plasmid is digested with this restriction enzyme, how many
fragments will be generated?
Note: in order to get to the right answer please draw.
3.
please answer all corectly. if you don’t know the
answers please refrain from answering this
question.thanks
Using the restriction map below, draw the bands you would expect to see on an agarose gel (after electrophoresis) if you digest the DNA with EcoRI alone, Pstl alone, and EcoRI+Pstl. How far are EcoRI and BamHI sites located on the plasmid? Submit your answer in kilobases. BamRLEcoRI EcoRI 6.0 kb 6.0kb 4.0 kl 2.0 k
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