(10 points) DNA Replication (10 points) Using the figure and accompanying legend below, explain how it was shown experimentally that small fragments (okazaki fragments) are present in the region of the replication fork. Figure () The type of data obtained by alkaline sedimentation of pulse-labeled ,-、Total DNA NA and pulse-chased DNA Total DNA is the sum of the nonradioactive and radioactive DNA. as might be indicated by the optical absorbance. The s value of the sedimenting material increases from right to left. (b) The location of radioactive DNA (so lid Puise lin at the time of pulse-labeling and after chase. The radioactive molecules present in alkali are shown. Pulse-chase / Tube bottom Direction of sedimentation 2. (10 points) Question on bond types. amino acid side chains and protein stnucture. Freifelder used the diagram below to illustrate how various non-covalent bonds cooperated to hold a polypeptide in a given three-dimensional configuration. Using this as a base, discuss the properties of each bond (numbered 1 – 6) and give specific examples of amino acids that would be involved in each bond type. Note: Draw structures of all amino acid side chains you used as examples. This is an essay problem with many points, so be thorough and complete in your answers. 3 Negatively charged amino acids H bonds lonic bonds. Stacked rings Hydrophobic cluste Van der Waals bonds A hypothetical polypeptide chain showing attractive and repulsive Interactiona.