2. During an analysis of a promoter you identify six sites (shown below) that alter the expression of the promoter when they are deleted. ACGGAG CGTGG AGGAAG AGGCAC ACGCAC AGGGAC (a) Since the number of sites is small, we will include pseudocounts distributed according to the background nucleotide frequencies in the genome. Assume you are working in a genome where %A %T 20%, and %G %C 30%. The sum of all pseudocounts per position should be 1. What are the pseudo-count values for each nucleotide? pseudocounts of A TE0.2 pseudocounts of C G 0.3 (b) Fill in the nucleotide count matrix, N(b,i) for this multiple alignment, including pseudocounts Count matrix (includin pseudocounts): 1 4