4. (25 pts). You are interested in the genes that respond to exe carbon soures that ar yo minimal medium. Your bacteria cannot utilize chocolate isolated a large number of Mud-lae transcriptional fusiof Centiied on minimal medium with excess chocolate, and Lac in are interested in the original set of chocolate-induced Mud insertion mutantMud-laa transcriptional and translational control of chod by then fusions to choA and then assaying the effect of adde excess chocolate added to as a carbon Sour are You have that are Lac ranscriptional fusion insertion mutants e of chocolate. You regulation of a the slational cular gene, chod, which was identified from youi gene, You u decide to compare t translational fusion. insertion mutants, what oa do you expect to be Lac in the presence of chocolate (a) For the isolation of the chod:Mud-lac translational fusion solate 100 chod:Mud-lac translational fusion insertion mutants, but none will grow pho insertion mutants. Of these 100, 15 are PhoAt in the presence of on lactose in the choA::Tn5 chocolate presence of chocolate. You do another experiment and isolate 100 (b) Account for the fact that 0/100 choA:Mud-lac translational fusion insertion mutants failed to grow on lactose in the presence of chocolate. (c) What simple experiment might allow you to test your hypothesis?