Please answer #5 given the answer for #4. Thank you!!

5. Intrigued by the theoretical possibilities above, you decide to check if such SRY mutants exist in the human population. You are particularly intrigued with the potential for a mutation in the promoter. So you gather a cohort of patients in which there is a deficiency in SRY function. A. (4 pt) What will the genotype (in terms of sex chromosomes) and the phenotype (external appearance) of these patients be? Genotype Phenotype B. (10 pt) Describe one possible strategy to clone the SRY locus from these patients including what technique (s you would use. Assume that the location of the gene on the Y chromosome is known, so no mapping is required. Make sure to draw a schematic that specifies where any primers for PCR or probes (for Southern blot or colony hybridization) will be situated relative to the gene, or which part of the resulting protein will be recognized by an antibody, depending on the techniques proposed. If you make any libraries, specify how they were made (origin of the genetic material, restriction enzyme used, etc) More than one successful strategy exists, but whichever you use, make sure that you clone the non-coding parts of the gene such as the promoter. rest (10 that only 5% of your has a mutation in the promoter. The have missense mutations in a part of the protein that is NOT involved in binding. You hypothesize that these mutations somehow interfere with the localization of this protein to the nucleus. Describe how you would go about finding out if there is a problem with the localization of the SRY protein in these patients.