A polypeptide has the sequence:
Leu-Leu-Trp-Tyr-Ser-Glu
a.
Calculate the molecular
weight of the polypeptide.
b.
Assuming a molar absorptivity for Trp and Tyr of
3400M-1cm-1 and
1400M-1cm-1, respectively, estimate the
extinction coefficient for this peptide at a wavelength, l, of 280
nm.
c.
A solution of
this peptide is placed in a cuvette with a 1 cm pathlength and its
absorbance is found to be 1.3 at 280 nm. What is the concentration
of the polypeptide in mg/ml?





