. Auxotrophic strains A-D are compared with a wild type strain
that can synthesize a nutrient required for growth. In the wild
type strain there is a single starting compound, followed by three
intermediate compounds, before the final nutrient is produced. All
mutant strains have a block somewhere I the pathway, but because
they have different blocks, they are able to survive if different
intermediate compounds are provided. A “+” means growth if the
given compound (1-5) is provided and “-“ means no growth.
|
Strain |
1 |
2 |
3 |
4 |
5 |
|
A |
– |
+ |
– |
– |
– |
|
B |
– |
+ |
– |
– |
+ |
|
C |
+ |
+ |
– |
– |
+ |
|
D |
+ |
+ |
– |
+ |
+ |
|
Wild type |
+ |
+ |
+ |
+ |
+ |
Determine the order of the intermediates and also indicate
where, in the pathway, each strain A-D is blocked. 2 pts
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