Challenge question You have cloned a fragment of MolBio333 enzyme coding region into the unique EcoRI site of the pUC18 pLinker. You now need to prepare a large quantity of radiolablelled coding DNA to probe a Suthern blot containing Notl (an 8-cutter) digested chromosome 12 DNA on which the MolBio333 gene resides. If you identify & sequence the whole gene, you have a chance of publishing your findings in Nature and wrap up your PhD In your freezeer you have gamma P32-labeled ATP and every enzyme you could ask for Starting with a bacterial clone that carries your recombinant plasmid, how do you go about making your radiolabeled DNA probe? GA ATT C CTT AAG EcoRI recognition site Poly linke lacz amp ac promoter 2.7 kb pUC18 vector Now, suppose someone bad used up your gamma-P ATP and all you have left is the alpha- P ATP. What new series of biochemical reactions could you design to end up with a radiolabeled probe?