Class Date Chapter 13 Quiz Indicate the answer cholce that best completes the statement or answers the question 互! ng sites C”poly-linkers”) in most commercially available plasmids offer all the following advantages over the earlier plasmids used in genetic recombination except a. They can be used with a wide variety of restriction enzymes. b. They assure that only the desired DNA will be inserted into the plasmid e. They enable easy control of the direction of insertion of the gene of interest d. They are usually adjacent to any necessary promoters for gene expression. e. All of these are advantages Exhibit 13A This is a map of the pBR 322 plasmid, numbers on the map refer to the base where the site is located 29 eokV 185 All 651 Aay Hn 3056 ORI Annu HgiE1I 2295 2. Refer to Exhibit 13A. Ifa recombinant plasmid were obtained inserting DNA into the Aill site, the recombinant plasmid would lack which of the following properties? a. It can replicate autonomously b. It will be resistant to the antibiotic tetracycline c. It will be resistant to the antibiotic ampicillin. d. It will possess at lest two promoter sites. e. All of these are true for the recombined plasmid. Pa