Cultural Methods and Physiological Methods: Explain the difference between differential and selective media Understand the biochemical basis of the tests we performed in lab Know how to interpret the results in the different media and test What media is used to perform a test? What are the Durham tubes used for? Are there any indicators in the media? What is Phenol red and why is it used? What are the reagents used in the test? What are the products after the test is performed? Differentiate the growth patterns between aerobes, facultative aerobes, and anaerobes In FTM media what is resazurin? What does it detect? Know the test to determine the ability of the bacteria to degrade or hydrolyze starch, tryptophan, urea, and phenylalanine Urea RDS HEA m-staph broth MSA plate EMB plate Blood agar Bile esculin Catalase Nutrient Gelatin FTM Glucose Broth Lactose Broth Sucrose Broth Mannitol Broth Nitrate Broth Tryptone Broth MR-VP Test Starch Hydrolysis Kligler Iron Test Simmon’s citrate Test Phenylalanine Test Litmus Milk Gram staining