Design the forward and reverse primers to the following genes.
Write answers in the 5′->3’ direction. Primers are usually ~20
nucleotides with Tm’s around 45-60°, but only design them to be 6
nucleotides long to
reduce work. Calculate the Tm of each primer, and determine what
annealing temperature you would use in
PCR. You do not need to look for a start codon, as this is a random
sequence of DNA.

5’-GTACTAGTAGACCGTATGCCTCAGTA-3’

Forward: ______________________ Tm: _______
Reverse: ______________________ Tm: _______
Annealing temperature________________