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Question: Design the forward and reverse primers to the following genes. Write answers in the 5′ rightarrow…

by | Dec 5, 2023 | Posted Questions

Answer below Genetics related question( 12,13,14,15) with brief
understanding.

Question: Design the forward and reverse primers to the following genes. Write answers in the 5' rightarrow...

Design the forward and reverse primers to the following genes. Write answers in the 5′ rightarrow 3′ direction. Primers are usually ~20 nucleotides with Tm’s around 45-60 degree, but only design them to be 6 nucleotides long to reduce work. Calculate the Tm of each primer, and determine what annealing temperature you would use in PCR. You do not need to look for a start codon, as this is a random sequence of DNA. 12. 5′-GTACTAGTAGACCGTATGCCTCAGTA-3′ Forward: Reverse: Annealing temperature: Tm: Tm: 13. 5′-GCTGTAAGAGGATTTACTACCGTCCA-3′ Forward: Reverse: Annealing temperature: Tm: Tm: 14. Design the two primers (6 nucleotides), and add an EcoR1 cut site to the forward primer (the 6-letter sequence), and BamH1 cut site to the reverse primer. Look up the cut site sequence. Label the restriction enzyme cut sites and add two overhang bases. Cut sites are included in the Tm. 5′-CTATGAGGTCCTGCGTTAGTGTTACC-3′ Forward: Reverse: Annealing temperature: Tm: Tm:

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