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Question: Finding genes in double stranded DNA Below is a region of DNA. 5’-ttaccgataattctctctcccctcttcc…

Finding genes in double stranded DNA

Below is a region of DNA.

5’-ttaccgataattctctctcccctcttccatgattctgattaaagaaggcgagaacgaaactatttgttaatacc-3’

1) What is the nucleotide composition of this DNA
fragment?
2) Draw the other strand (don’t forget the orientation), then
indicate all 6 READING FRAMES.
3) Identify all the possible OPEN READING FRAMES (ORF): search for
start (ATG) and stop codons (TAA, TAG, TGA) on each individual
frame.
4) How many ORFs are found on this fragment?
5) What is the frame of the longest ORF?
6) How many codons in this ORF?
7) How many Amino acids in this ORF?
8) Using the one letter code for amino acid, translate the longest
ORF.
N-________________________________________________-C
Assuming this fragment of DNA is from a bacteria, what needs to be
found upstream/downstream this sequence to make it a potential
genes?

Please don’t just answer one question. answer all the related
questions neatly and easily. I’ll give a good rate for your hard
work.

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