*for a plaque Assay a virus Titer lab*
1. in this exercise there must be enough bacteria
inoculated to produce a lawn of growth. why is this important?
2. consider the adsorption step. how might the results
be altered if you had skipped the absorption phase?
how might the results be altered if you extended it?
3. consider the water broth. why was it set at 50
degrees Celsius?
what might be some consequences of raising it’s temp? of lowering
it?
4. considar the soft agar. why was soft agar used for
the overlay?
what would you expect to see if standard nutrient agar or
trypticase soy agar had been used instead?
thank you for helping.