Give an example of artificial or natural genetic transformation Is a plasmid part of chromosomal DNA? What 2 genetic traits does the p-Glo plasmid used in lab encode for? Cells must first be broken apart before DNA can be extracted What was the lysing agent using in lab to accomplish this? The DNase enzymes will degrade DNA and therefore must be deactivated if a successful DNA extraction is to be completed. What ion is required for DNase to function? What substance was used was used in lab to interrupt the activity of the DNase enzyme? How did it do this? Why was bacterial culture heated to 65 degree Cin the hot plate? What would happen if it was heated above 80 degree C? What was the purpose of using ethanol (EtOH) in the DNA extraction? Why is DNA soluble in water but not ethanol? What was CaCl_2 used to do in the bacterial transformation experiment? What was the purpose of placing the cells in the 42 degree C waterbath? Be able to list all the components required in order to perform a bacterial transformation (ie. Bacteria sample, plasmids, perforating agent (CaCl_2), growth medium, etc.) What was the antibiotic that was used in the culture plates? If the sugar arabinose acts like a switch that will turn on the green fluorescent protein gene, would you expect the bacterial cells that contain the plasmid for GFP to fluoresce when grown on a plate that has no arabinose? Be able to predict the results of the experiment: What would happen if you inoculated an LB plate with bacteria with the p-Glo plasmid? What would happen if you inoculated an LB/AMP plate with bacteria with the p-Glo plasmid, and without? Be sure you read through the entire lab protocol and understand it. There may be some questions on the quiz to which the topics were not expounded upon in lab. If you are unclear about any concepts please ask! Do not wait until the night before the quiz to ask questions if you have any. We will perform the data collection and analysis that is covered in the handout next week.