Given the structure below, please answer figure DNA or diesterase is an enzyme that accompanying and to Assume that the breaks the covalent bond that connects the pi to which carbon on the is digested with spleen die To which LA To sugar is the phosphate eow A) DNA: 3′ end, T. By RNA, y end. A. D) DNA. 3 end. A. 3 ERNA 3′ end, T DNA, end A.S 42) Assume that a plasmid (ei is 3200 base pairs in length and has restriction at the sites following locations: 1400, 2600, Give the expected sizes the restriction inagments digestion of A 400, 800, 1000 (2 these) B) 4000, 1200, 1600 C) 300, 700, 2200 D) 700, 400, 1400, 2600 E) 300, 700, 1000, 1200 43) Assume that you have cut ADNA with the restriction enzyme Hindllle You separate the fragments on agarose gel and stain the DNA with ethidium bromide, You notice that the intensity of the stain is less in the bands that have migrated closer to the What explanation below is not correct for this finding? A) the smaller fragments bind less stain B) the smaller fragments migrate toward the pole C) the smaller fragments migrate more away from the origin than larger fragments which are near the origin D) the larger fragment stains more than smaller fragment 44) List two especially useful characteristics of cloning vectors. A) high copy number and antibiotic resistance gene(s) B) virulence and lysogenicity C) ability to integrate into the host chromosome and then causing alytic cycle D) nonautonomous replication and transposition E) reverse transcriptase and ligase activities 45) Over the years, sophisticated plasmid vectors have been developed for use in recombinant DNA technology. Point out the feature below that has not been introduced in particularly usefu Vectors. A) small size to allow large inserts B) low copy number C) large numbers of unique restriction sites (multiple cloning sites) D) variety of selection schemes (pigmented colonies, antibiotic resistance)