Graph by hand.Graph without using graphing software, graph the
inverted data correctly on Lineweaver-Burk graph.
(AC) is the is ready to transmit again. In studying the rate of neuron impulse tr (leftmost column), you obtain the initial rate data ⅓ below, second column on the leh Repeating the experiments, you first add ammonia solution (NH) to AC. On ancther replication, you add a drug called edrophonium to AC. For these, you obtain the rate data in the corresponding columns IAC (mmol L) AC on Vo (mmol AC min) Vo(mmol AC min) Vo(mmol AC min) 4.76 1.5 2.78 2.86 2.5 3.0 8.33 9,09 9.72 10.26 6.25 4.0 5.0 8 33 1. Draw a graph of the three sets of data by ploting Vo versus ISI on a standand kinetic graph, in which [S] is, sequentially, [AC] only, [AC]+ NH, and [AC+ edrophonium, ic, don draw them on separate graphs, instcad, superimpose these three plots on the same graph for comparntive 2. Invert all of the [S] and V, data, and depict the inverted data on a table similar to the one sbove, on a separate shcet of paper (remember to invert the units, too). Then graph the three sets of inverted data using rk plot of 1o versus MS], in which VIS] is for, sequentially, AC] only. IAC]+ NH and [AC] + edrophonium. Again, superimpose the three plots on the same Linewearver Burk graph for comparative purposes 3. Estimate the value of Vanse of AC inactivation for each of the three reactions, ie, JAC) only, IAC) with NH, and IACI with edrophonium. Report these values on a Which of the two graphs did you actually use to estimate Vax7 Why? Also, depict and label the interpretation of Vx for each drew for question 1 and the Lineweaver-Burk graph you drew for question 2 three reactions on both the Michaclis-Menten o vx ISI graph you 4. Estimate the value of Km of AC inactivation for cach of the three reactions ie, JAC] only, [ACI with NHA, and IAC] with edrophonium. Report these valacs on a separate sheet of paper from the gapis Which of the two graphs did you actually use to estimate Ka? Why? Also, depict and label the gaphial interpretation of K, for each of the three reactions on the two graphs 5. What type of inhibitor is NHa7 What type of inbibitor is edrophonium? In cach cas, based on the





