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Question: How is the gene for β-galactosidase used in the context of cloning with plasmids? Question 3 opt…

by | Dec 5, 2023 | Posted Questions

How is the gene for β-galactosidase used in the context of
cloning with plasmids?

Question 3 options:

The gene is a promoter that is sensitive to the presence of the
sugar, galactose.

It is an origin of replication.

It is a cloning site.

It is a marker for insertion of foreign DNA into the
plasmid.

Question 4 (2 points)

A DNA library is

Question 4 options:

an orderly array of all the genes within an organism

a collection of vectors containing random segments of DNA

the collection of plasmids found within a single E. coli
cell

a collection of DNA fragments that collectively represents the
entire genome of an organism

Question 5 (2 points)

What enzyme is specifically needed in order to make a cDNA
library?

Question 5 options:

DNA helicase

RNA polymerase

Reverse transcriptase

Restriction exonuclease

Question 6 (2 points)

Why are “sticky ends” of DNA sticky?

Question 6 options:

Sticky ends of DNA are oppositely charged and electrostatically
attracted to one another

Sticky ends contain a single-stranded segment of nucleotides
that is complementary to a target fragment’s single-stranded
end

Sticky ends refers to the phosphate group on the end of the DNA
molecule being sticky to the next nucleotide.


Sticky ends bind to all other pieces of DNA where bases jut out
from the end, as a natural repair mechanism.

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