How is the gene for β-galactosidase used in the context of
cloning with plasmids?
Question 3 options:
The gene is a promoter that is sensitive to the presence of the |
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It is an origin of replication. |
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It is a cloning site. |
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It is a marker for insertion of foreign DNA into the |
Question 4 (2 points)
A DNA library is
Question 4 options:
an orderly array of all the genes within an organism |
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a collection of vectors containing random segments of DNA |
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the collection of plasmids found within a single E. coli |
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a collection of DNA fragments that collectively represents the |
Question 5 (2 points)
What enzyme is specifically needed in order to make a cDNA
library?
Question 5 options:
DNA helicase |
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RNA polymerase |
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Reverse transcriptase |
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Restriction exonuclease |
Question 6 (2 points)
Why are “sticky ends” of DNA sticky?
Question 6 options:
Sticky ends of DNA are oppositely charged and electrostatically |
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Sticky ends contain a single-stranded segment of nucleotides |
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Sticky ends refers to the phosphate group on the end of the DNA |
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Sticky ends bind to all other pieces of DNA where bases jut out |