i need help identifying my Unknown gram negative bacteria based
on these tests

thiobroth

Broth was cloudy with thicker growth and several white clumps
floating near the top.

Temperature Plate

At 4˚ there was very little growth on the plate.

At 25˚ there was a large amount of growth.

At 37˚ there was a large amount of growth.

At 50˚ there was little growth on the plate.

Osmotic Pressure

At 2 there was some growth.

At 5 there was little growth.

At 8 there was little, “stringy” growth.

At 11 there was little growth that looked like small fibers.

MAC, HE, and EMB plates

No growth on any of the plates.

Oxidase Test

Negative oxidase test

Oxidation Fermentation

Without mineral oil there was a large amount of orange growth
throughout the tube.

With mineral oil there was a large amount of orange growth
throughout the tube.

Phenol Red Fermentation TestLactose- No fermentation

Orange/red with no bubble

Glucose- fermentation with acid end products, no gas
produced

Yellow with no bubble

Sucrose- No fermentation

Orange/yellow with no bubble

Trehalose- fermentation with acid end products, no gas
produced

Yellow with no bubble

Lipase Test

Clearing

DNAse

Cloudy, no clearing

L-lysine Decarboxylation

Yellow

L-orthinine           

Purple

Citrate      

Growth, no color change

Gelatinase

Still gel form, did not liquefy

Urease

Orange/yellow

Motility

Red diffusion growth

TSIA

Red with growth

SIM

Growth, no black spot

Phenylalanine Deaminase

No color change

ONPG      

Yellow

Nitrate Test

Red with reagents added

Red with zinc is added

Indole

Negative

MR

Positive

VP

Negative

7.14 Flowchart for Identifying Indole-Negative,
MR-Positive, VP-Negative, Citrate-Positive or –Negative
Enterobacteriaceae

Indole-Negative, MR-Positive, VP-Negative

Methyl Red test is used to test for organisms capable of
performing a mixed acid fermentation.

Voges-Proskauer test is used to identify organisms that are able
to ferment glucose.

Shigella sonnei is capable of performing a mixed acid
fermentation but is not capable of fermenting glucose.

Glucose Fermentation-Negative

This tests the ability of the organism to ferment glucose.

My unknown was not able to ferment glucose.

Esculin Hydrolysis-Negative

Bile Esculin Agar is an undefined, selective, and differential
medium. It tests for the production of D-glucose and esculetin that
can occur under acidic conditions.

Based on this description my bacteria would no turn black
telling us that it does not produce D-glucose or esculetin.

L-Ornithine Decarboxylase-Positive

Decarboxylase is a media that includes an amino acid and is used
to differentiate between organisms in the family
Enterobacteriaceae and other gram-negative rods.

My test turned purple meaning that in is lysine decarboxylase
positive.