If a student accidentally plated his entire transformation on a
Nutrient Agar plate, is there any way she could determine which of
the colonies were transformed with the plasmid?
A.All the answer choices are correct. B.She could isolate and
sequence DNA from several colonies to determine which ones are
transformants. C.She could make new cultures with the colonies in a
Nutrient broth treated with ampicillin and see which cultures grow.
D.She could streak the colonies on Nutrient Agar + amp plates and
see which ones grow.
If a student accidentally plated his entire transformation on a Nutrient Agar plate, is there any way she could determine which of the colonies were transformed with the plasmid? A. All the answer choices are correct. She could isolate and sequence DNA from several colonies to determine which ones are transformants. C. She could make new cultures with the colonies in a Nutrient broth treated with ampicillin and see which cultures grow D. She could streak the colonies on Nutrient Agar+ amp plates and see which ones grow.
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