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Question: If one were to move the lac operator to the far end of the operon (past the transacetylase gene),…

by | Dec 5, 2023 | Posted Questions

Question: If one were to move the lac operator to the far end of the operon (past the transacetylase gene),...

If one were to move the lac operator to the far end of the operon (past the transacetylase gene), which of the following would likely occur when the cell is exposed to lactose? A. The inducer will no longer bind to the repressor. B. The repressor will no longer bind to the operator. C. The operon will never be transcribed. continuously. D. The structural genes will be transcribed continuously. E. The repressor protein will no longer be produced. If one were to move the repressor gene (lac 1), along with its promoter, to a position at some several thousand base pairs away from its normal position, which will you expect to occur? A. The repressor will no longer be made. B. The repressor will no longer bind to the operator. C. The repressor will no longer bind to the inducer. D. The lac operon will be expressed continuously. E. The lac operon will function normally. Why are yeast cells frequently used as hosts for cloning? A. They easily form colonies B. They can remove exons from mRNA C. They do not have plasmids D. They are eukaryotic cells E. Only yeast cells allow the gene to be cloned To introduce a particular piece of DNA into an animal such as of a mouse, you would find more probable success with which of the following methods? A. The shotgun approach B. Electroporation followed by recombination C. Introducing a plasmid into the cell D. Infecting the mouse cell with a Ti plasmid E. Transcription and translation Which was developed by a British researcher and causes DNA sequences to be transferred to membrane and identified with a probe? A. Southern blotting B. Northern blotting C. Western blotting D. Eastern blotting E. RT-PCR

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