Indicate which specific step of transcription is disrupted in the following mutants: In mutant 1, the RNA polymerase can bind to the promoter but it does not stay bound; in other words, RNA pol is only able to bind reversibly. Mutant 2 makes short RNAs that correspond to the first 10 nt of the 5’UTR, but cannot make full length transcripts. Mutation in which specific cis elements would lead to the above two phenotypes. Indicate what the nature of the mutation is, what the cis element is, and why this type of mutation would lead to the indicated phenotype. Mutation in which specific trans factors would lead to the above two phenotypes? Indicate what the nature of the mutation is, what the trans factor is, and why this type of mutation would lead to the indicated phenotype.
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Question: Indicate which specific step of transcription is disrupted in the following mutants: In mutant 1…
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