LAB MODULE: BASIC MICROSCOPY – STUDY HELP
The information in this document is helpful in preparing
for the Basic Microscopy Pre-Lab Quiz
on Canvas and the Basic
Microscopy quiz on Late Nite
Labs.
Instructions:
• Go to your Late Nite Labs course site and
click on the Basic Microscopy link. The lab manual content will
appear on the right side of the screen.
• PRINT and READ all of the information found in the
Basic Microscopy lab manual.
• Use the information to answer the following
questions.
Questions
• Fill in the remainder of the chart.
|
Objective Lens |
Objective Magnification |
Ocular Magnification |
Total Magnification |
|
Scanning |
4X |
10X |
40X |
|
Low Power |
10X |
10X |
|
|
High Power |
40X |
10X |
|
|
Oil Immersion |
100X |
10X |
• Do all organisms consist of more than one
cell?
• What is the structural and functional unit of
living things? In other words, what is considered the basic
building block of LIFE?
• What three structures are found in the cells of
ALL organisms, both prokaryotic and eukaryotic? You may have to
look at the pictures of the cells in the Basic Microscopy
lab manual content to find all three structures. Note: plasma
membrane = cell membrane
• List the major differences in prokaryotic cells
and eukaryotic cells? Include the location of genetic material in a
eukaryotic versus prokaryotic cell.
• List the organelles found in a eukaryotic cell
that are not found in a prokaryotic cell. You may have to look at
the pictures of the cells in the Basic Microscopy lab
manual content to find all of these.
• In eukaryotic cells, genetic information is found
in the form of DNA. Is this true for prokaryotic cells?
• Are flagella and cell walls unique to eukaryotes?
Why or why not?
• The __________is the part of a cell that allows
the movement of substances into and out of the cell and protects
the cell from its environment.
• The__________is a substance that contains all of
the dissolved and suspended ions and other molecules needed to keep
the cell alive.
• A__________is an organelle that serves as the site
of photosynthesis in a eukaryotic cell.
• Some organisms have cells that contain
a__________that gives the cell a more structured shape and
strengthens the cell by osmotic pressure.
• Describe dark field microscopy.
• Describe phase contrast microscopy.
• Why do scientists use dark field microscopy and
phase contrast microscopy when studying prokaryotic cells?
• How does phase contrast microscopy help scientists
visualize difficult specimens?
• Fill in the remainder of the chart. The organisms
studied during this lab are: Amoeba, Spirogyra,
cardiac muscle, and bacteria.
| Structure | Function | Organisms Studied |
| Plasma Membrane |
Amoeba, Spirogyra, cardiac muscle, and |
|
| Cytoplasm | ||
| Nucleus |
Contains the genetic material of a eukaryotic cell |
|
| Chloroplast |
Spirogyra |
|
| Cell wall |





