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Question: Lactase, also known as beta-galactosidase, catalyses the hydrolysis of lactose to produce glucose…

by | Dec 5, 2023 | Posted Questions

Question: Lactase, also known as beta-galactosidase, catalyses the hydrolysis of lactose to produce glucose...

Lactase, also known as beta-galactosidase, catalyses the hydrolysis of lactose to produce glucose and galactose from milk and whey. Initial rate data below were obtained from experiments carried out to determine the kinetic parameters for the enzyme. (a) Using the initial rate data present (a) Lineweaver-Burk and (b) Hanes plots (b) From the two plots calculate: (i) the V_max (ii) the K_m (iii) k_2 given that E_o = 0.015 g^-1 (iv)Comment on the values of V_max, K_ and k_2 obtained using the Lineweaver-Burk and (b) Hanes plots

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