genomic library

restriction endonuclease

cDNA library

dideoxynucleotides

multiple cloning site (MCS)

hybridization

embryonic stem cells

reverse transcriptase

western blot

polymerase chain reaction

DNA ligase

recombinant DNA

restriction sites

knockout transgenic

vector

A region of a plasmid that contains a number of unique
restriction sites.

Catalyzes the formation of a phosphodiester bond between
adjacent PO4 and -OH groups of nucleotides.

Specific DNA sequences that are cleaved by particular
restriction enzymes.

A single DNA molecule made from two different sources.

A piece of DNA that carries a recombinant DNA molecule and can
replicate in a host cell.

Enzymes capable of cleaving DNA at specific sequences.

Allows the amplification of a single DNA fragment from a small
amount of a complex mixture of DNA.

Acts as a chain terminator when incorporated into the DNA
because it lacks an OH at the 3′ position.

A technique in which proteins are separated by electrophoresis
and probed with a specific antibody.

The inactivation of a specific gene to assess the effect of the
loss of its function in an organism.

Cells derived from early embryos that can develop into different
adult tissues.

An enzyme isolated from retroviruses that can make cDNA from an
mRNA template.

A collection of many different stretches of DNA in a vector that
together represents the entire genome of an organism.

Annealing of a complementary single-stranded probe to identify
specific DNA’s in complex mixtures.

A collection of many different types of DNA that each represents
the genes expressed as proteins in many different tissues or
cells.