multiple times.
Molecular smaller bands you see in the double digest should add up to the bands seen in the A digest alone. Determine which bands of the double digest come together to form the small A restriction fragment. a. b. Determine which bands of the double digest make up the large A fragment. Draw the two A restriction fragments in the space provided, and in- dicate where the B restriction sites fall on the fragments Indicate the size of the fragments that are produced in the double digest c. d. Do the same analysis on the B fragments Compare the double di- e. Draw the B restriction fragments in the gest with the single B digest where the A restriction sites fall on the fragments Indicate the size of the fragments that are produced in the double digest f Align the fragments from the preceding analysis. You will notice from your drawings that there are double digest fragments of the same size These fragments are the key to solving our puzzle because they nep- resent overlap. If we align the large B fragment and the large A frag- ment, we find that we can match up restriction sites (Figure 10) g Continue to align fragments. As shown in Figure 10, the align- ment is used to draw out one longer fragment By continuing to





