Notice that the genes for lysogeny are clustered in an operon on
the “left” side of the phage plasmid, while the genes for lysis are
clustered in an operon on the “right” side of the plasmid.
At the beginning of each infection, transcription begins at two
“early” promoters.
“Leftward” genes
Early promoter PLcontrols leftward
transcription of early genes, beginning with the N gene.
The N protein plays an anti-termination role in transcription from
PL. Leftward transcription is also enhanced by
binding of the cII/cIII complex to the
PRE promoter, transcribing the cI
gene. The cI protein enhances transcription of “leftward”
genes and represses transcription of “rightward” genes. Genes under
control of PL are involved in the integration
of viral DNA into the bacterial chromosome.
“Rightward” genes
Early promoter PR controls rightward
transcription of genes, beginning with cro. The cro
protein enhances the transcription of the genes that are rightward
of the PR, and prevents transcription of the
leftward gene, cI. Genes under the control of
PRare involved in the synthesis of head and
tail proteins as well as proteins that lyse the host cell.
Based on this information, determine the phenotype of
each phage mutation. (PLEASE SELECT WHICH GOES INTO WHICH
CYCLE)
Regulator of Regulator Arci, and int gene of ci gene expression Phage DNA Regulator of replication proteins early genes X repressor cro Stabilizer OP of oil protein N Regulator of Phage- cll late genes recombination Lysis o Pa origin of proteins proteins Excision ase replication (for excision from chromosome) -xis Cutting of Integrase int Nul DNA at (for integration A Terminase cos site for packaging into chromosome att Lamda phage 48,502 nucleotides Nu3 Genes for head proteins F11 and assembly I K L M Genes fortail proteins and assembly