Q1) M. Kreitman 1983 reported the following in 11 D.
melanogaster F/S polymorphic Adh haplotypes, DNA sequences of
diverse origin including indels were 2721 bases long and: (1) ‘ the
sequence of all 11 homologous chromosomes were unique; (2)
disregarding indels, and among nucleotides in introns and exons,
there were 9 different haplotypes, 8 were unique and 1 comprised 3
sequences. Within exons, 43 bases of 768 were polymorphic, and only
one accounted for the difference between Adh Fast (5 alleles) and
Adh Slow (6 alleles). Assume that this sample is characteristic of
the population. a) What does this data say about population:
polymorphism, heterozygosity, and haplotype frequencies? When
answering, carefully distinguish between the protein migration
allele and gene sequence allele . b) The following (k= 3) unique
DNA sequences (i, ii, iii) were found in 4 individuals. Allele (ii)
appeared twice. The total sequence length was 500 nucleotides.
Calculate nucleotide diversity, the proportion of differences for
all pairs (pij), fill in the following table and calculate
nucleotide diversity P: i) A…A…A…A…G ii) A…T…A…C…T
iii) G…A…A…A…T c) Compare (utility, theory, assumptions,
function, sensitivity etc.) 2 measures of nucleotide diversity P
(p) and q .