Suppose cells in an experiment had been labeled with green
fluorescent tubulin. At the onset of Anaphase B, you use your laser
to bleach a stripe across all of the microtubules on one side of
the spindle as shown by the dashed line. This does not hurt the
function of the microtubules in any way, but the bleached,
nonfluorescent subunits in the microtubules now serve to mark a
fixed location relative to the (+) and (-) ends.
1) Label one of each of the following: kinetochore MT, astral
MT, polar MT. Indicate (+) and (-) ends.
MT, polar MT. Indicate (+) and (-) ends.
2) As the cell progresses through anaphase B, do the bleached
spots get closer to, further from, or stay the same distance from
the spindle pole they are embedded in? Why? Do they get closer to,
further from, or stay the same distance from the plasma membrane?
Why?
spots get closer to, further from, or stay the same distance from
the spindle pole they are embedded in? Why? Do they get closer to,
further from, or stay the same distance from the plasma membrane?
Why?
pl. membr. spindle
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