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Question: Suppose you discover a novel process in a little-studied cell type in which a chemical insult cau…

Suppose you discover
a novel process in a little-studied cell type in which a chemical
insult causes an unknown organelle to undergo energy-dependent
movement from near the MTOC to the plasma membrane. To probe the
cytoskeletal elements required for this movement, you add an
actin-depolymerizer, latrunculin A. This does not affect the
initial movement of the organelle away from the MTOC, however, the
organelle stops short of reaching the plasma membrane. You next try
the microtubule-depolymerizer colchicine. This prevents any
movement of the organelle away from the MTOC in response to the
stimulus.

1) What do
these results tell you about the identity of the cytoskeletal
elements involved and the identity of the required motor protein(s)
over the course of the movement?

2) Predict the result you would get if you added both
latrunculin A and colchicine at the same time. Would the resulting
phenotype be a combination of adding each drug alone, would one or
the other drug’s effects predominate, or would they cancel out?
Why?
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