The direction of protein band migration in Western blotting is from anode (positive pole) to cathode (negative). True False Most proteins have positive charge above pH 7.0. True False Which of the following is the proper assembly sequence in Western blotting? Important Assume the negative electrode is on the bottom. From bottom to top Fiber pad, filter paper, nitrocellulose membrane, gel filter paper, fiber pad From bottom to top Fiber pad, nitrocellulose membrane, filter paper, gel, filter paper, fiber pad. From bottom to top: Fiber pad, filter paper, gel, nitrocellulose membrane, filter paper, fiber pad. From bottom to top: Fiber pad, nitrocellulose membrane, filter paper, gel, filter paper, fiber pad.