The genome of Rickettsia prowazekii was reported in
1998, and has 1,111,523 bp. As a bacterium, R. prowazekii
has circular duplex DNA.
1. The G+C content is 29.1%. Give the expected percentage for
each nucleotide.
2. Calculate the linking number if the DNA is relaxed and we
assume 10.5 bp per turn.
3. DNA is commonly observed to have a superhelix density s of
-0.06. What is the observed linking number? If the DNA is not
supercoiled (ie, W=0), what happens to the number of bases per
turn? If the DNA instead maintains 10.5 bp/turn, how many
supercoils are formed? What must W be?
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