The process of creating mRNA from
DNA is known as transcription. Using the mRNA to then create a
protein is called translation. We will be practicing both of these
steps of the central dogma using small proteins. Remember
that when mRNA is formed, it complements DNA. C&G are always
paired; however, RNA uses the base U instead of T, so that will be
paired with A in the mRNA strand. DNA and RNA are always read in
the 5’ to 3’ direction, and complementary strands will be
anti-parallel. A table of RNA to amino acids has been included.
Angiotensin II is an 8 amino acid
(aa) peptide that works to help increase blood pressure when there
is low blood volume. It is produced from a much larger, inactive
peptide by activating a restriction enzyme that cleaves off the 8
aa sequence.
Angiotensin II: 8 a.a. ->
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
1.Using the table above, give a
possible mRNA sequence that would code for this peptide.
(2pt)
2.Explain why the sequence you wrote
above is not the only mRNA sequence that could code for angiotensin
II. (2pt)
Second base Phe UCA Ser UAA Stop UGA Stop A UUA. UUG UCG UAG Stop UGG Trp G OGU His CUC. CCC CAC CGC Leu Arg CUA CCA CAA. CGA A Gln AUTU ACU AAU AGU U E AUC Ile Asn Ser C ACC AAC AGC Thr AUA ACA AAA AGA. |A Lys Arg AUG Met ACG AAG AGG Guo Goc GAC Asp GGC Val Ala Glu GUG GCG GAG GGG