The restriction enzyme BglII has the recognition sequence (5′
A*GATCT 3′) and cuts between the A and the G as indicated by the
asterisk. DNA cut by which of the following restriction enzymes
(given with their recognition sequences and cut sites) could be
ligated to a piece of DNA cut with BglII?
Question 14 options:
|
XbaI (T*CTAGA) |
|
|
HindIII (A*AGCTT) |
|
|
BamHI (G*GATCC) |
|
|
PvuI (CGAT*CG) |
When cloning a gene into a plasmid, if the gene is inserted into
the lacZ gene the bacteria that takes up plasmid with a foreign
gene inserted into it will turn blue when grown on X-gal.
Question 12 options:
| True | |
|
False |
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