Tube
Enzyme/s used to digest pBR322
A EcoRI
B BamHI
C PstI
D EcoRI
+ BamHI
E BamHI
+ PstI
F PstI
+ EcoRI
Ladder 1kb
| Fragment size (bp) | Log of fragment size (bp) |
| 10000 | 4.00 |
| 8000 | 3.90 |
| 6000 | 3.78 |
| 5000 | 3.70 |
| 4000 | 3.60 |
| 3500 | 3.54 |
| 3000 | 3.48 |
| 2500 | 3.40 |
| 2000 | 3.30 |
| 1500 | 3.18 |
| 1000 | 3.00 |
| 750 | 2.88 |
| 500 | 2.70 |
| 250 | 2.40 |
| Lane | Actual Fragment size (bp) | fragment size (bp) | Log of fragment size (bp) |
| A | 4361 | 0 | 3.64 |
| B | 4361 | 0 | 3.64 |
| C | 4361 | 0 | 3.64 |
| D | 3984 | 377 | 3.60 |
| E | 3236 | 1125 | 3.51 |
| F | 3613 | 748 | 3.56 |
Please discuss the results (in details) obtained above and do
mention why you can see more than one band in the uncut plasmid
lane.
see more
1 kb DNA Plasmid A F Ladder pBR322 Undigested And Digested fractions 4.3 kb 1.1 kb 0.7 kb 0.3 kb
ScholarMatic: Explanation & Answer
Your ready answer from a verified tutor is just a click away for as little as $14.99
Click Order Now to get 100% Original Answer Customized to your instructions!
