I need help with questions 4-5 please.

use the data table above to answer questions 1-3. 1. Which enzyme has the highest affinity for its substrate? How do you know? Which enzyme can convert the most substrate to product in a given period of time? How do you know? Which enzyme has the highest catalytic efficiency? How do you know? A biochemist is studying product formation over a given period of time. You’ll notice that he ran his luciferase enzyme assay (in triplicate) under varying concentrations of substrate (ie 5nM luciferin, 10nM luciferin, etc). RLU represents the concentration (in M) of product. Given the data in the attached excel spreadsheet, use linear regression to create a graph for each substrate concentration. In the graph, let the X axis represent time (in seconds) and the Y axis represent average product concentration (in M). Force your line to go through the origin. Note the equation of the best fit line. Paste the graph below. You should have one graph for each substrate concentration listed (i.e 5 plots on the same axes). Using graphing & the information from the graphs created in question 4, determine if the enzyme exhibits cooperative substrate binding. Provide your rationale.