What potential problems must be considered in producing human
insulin protein in bacteria?
Question 8 options:
|
Introns in the human gene will not be excised after |
|
|
The bacterial cell will be unable to post-translationally |
|
|
There is no way to get the bacterium to transcribe high levels |
|
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Both A and B represent problems. |
Question 9 (2 points)
Which of the following vectors has proven useful for introducing
genes into plants?
Question 9 options:
|
agrobacterium tumefaciens |
|
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retrovirus |
|
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adenovirus |
|
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bacteriophage lambda |
Question 10 (2 points)
The optimal temperature for Taq polymerase to extend primers
is
Question 10 options:
|
58°C. |
|
|
72°C. |
|
|
55°C. |
|
|
40°C. |
|
|
94°C. |
Question 11 (2 points)
In a polymerase chain reaction, 2 short 10-12 bp sequences of
nucleotides are involved in
Question 11 options:
|
copying the complementary strands. |
|
|
denaturing the complementary strands. |
|
|
annealing to each other to start the chain reaction. |
|
|
heating to separate the DNA ladder. |
|
|
priming for the DNA polymerase. |
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