What will the genotype (in terms of sex chromosomes) and the phenotype (external appearance) of these patients be? Genotype____ Phenotype____ B. Describe one possible strategy to clone the SRY locus from these patients including what technique(s) you would use. Assume that the location of the gene on the Y chromosome is known, so no mapping is required. Make sure to draw a schematic that specifies where any primers for PCR or probes (for Southern blot or colony hybridization) will be situated relative to the gene, or which part of the resulting protein will be recognized by an antibody, depending on the techniques proposed lf you make any libraries, specify how they were made (origin of the genetic material, restriction enzyme used, etc.) More than one successful strategy exists, but whichever you use, make sure that you clone the non-coding parts of the gene such as the promoter. C. You find that only 5% of your cohort has a mutation in the promoter The rest have missense mutations in a part of the protein that is NOT involved in DNA binding. You hypothesize that these mutations somehow interfere with the localization of this protein to the nucleus. Describe how you would go about finding out if there is a problem with the localization of the SRY protein in these patients.