Which of the following statements about PCR is/are FALSE? There may be more than one correct answer. A) PCR reactions contain DNA template, primers, deoxyribonucleotide triphosphates, and heat resistant DNA polymerase B) Each PCR cycle includes three steps: denaturation, annealing, and extension C) After each PCR cycle, the amount of DNA increases in a linear manner D) One limitation of PCR is that it requires prior knowledge of the sequence to be amplified E) PCR is a very sensitive technique and can amplify target sequences present low copy number F) The single-stranded DNA primers must anneal to opposite strands of template DNA at distant sites, with their 5′ ends directed toward each other In eukaryotes, activation of gene transcription depends on: A) trans-acting factors binding to cis-acting elements B) trans-acting factors failing to bind to cis-acting elements C repressor proteins binding to operator sites D) metabolites binding to cis-acting elements E None of the answer options are correct The spliceosome functions to: A) insert introns into mRNA sequences before translation. B) create noncoding sequences in genes to enhance gene stability C) remove introns from transcribed RNAs D) control translation, ensuring that only exons are translated E) inhibit transcription of noncoding DNA regions Which of the following is/are NOT a key structural property displayed by the heritable material? It may be necessary to circle more than one answer. A) It must be capable of being copied accurately B) It must contain the information necessary to form proteins and complex structures
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Question: Which of the following statements about PCR is/are FALSE? There may be more than one correct answ…
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