Which of the following statements about PCR is/are FALSE? There
may be more than one correct answer.

A) PCR reactions contain DNA template, primers,
deoxyribonucleotide triphosphates, and heat- resistant DNA
polymerase

B) Each PCR cycle includes three steps: denaturation, annealing,
and extension

C) After each PCR cycle, the amount of DNA increases in a linear
manner

D) One limitation of PCR is that it requires prior knowledge of
the sequence to be amplified

E) PCR is a very sensitive technique and can amplify target
sequences present in extremely low copy number

F) The single-stranded DNA primers must anneal to opposite
strands of template DNA at distant sites, with their 5′ ends
directed toward each other