( Hypothalamus D– Hormone 1 Short-loo negative feedback Long-loop negative feedback Antenor pituitary Hormone 2 Target endocrine gland Hormone 3 Target tissue -) A reduction in Hormone 2 in the bloodstream will result in an increase in Hormone 3. The target tissue produces a response that limits the production of Hormone 1 from the Hypothalamus The presence of Hormone 1 acts to negatively control the production of Hormones released from the Hypothalamus. A malfunctioning Anterior Pituitary gland will result in reduced concentrations of Hormone 3 in the blood